Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine] (Pre-adsorbed)

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Dot Blot: Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine] [NBP1-73629] - Dot Blot of Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine]. Antigen: Mouse IgG1. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications DB, FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Rhodamine
Format
Pre-adsorbed
Concentration
LYOPH

Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine] (Pre-adsorbed) Summary

Immunogen
Mouse IgG1 heavy chain
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
Application Notes
This product is designed for ICC/IF, FLISA and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.0 ml of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Alternate Names for Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine] (Pre-adsorbed)

  • IgG1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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