Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine]

Dot Blot: Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine] [NBP1-73629] - Dot Blot of Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine]. Antigen: Mouse IgG1. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Incubated for 1hr at RT; blocked for 60 min at RT.

• Reactivity: Mu
• Applications: DB, FLISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Rhodamine
• Concentration: LYOPH

Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine] Summary

• Immunogen: Mouse IgG1 heavy chain
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
• Application Notes: This product is designed for ICC/IF, FLISA and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water

Alternate Names for Rabbit anti-Mouse IgG1 Secondary Antibody [Rhodamine]

• IgG1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.