Goat anti-Guinea Pig IgG1 Secondary Antibody [HRP]

Product Discontinued

Goat anti-Guinea Pig IgG1 Secondary Antibody [HRP] Summary

• Immunogen: Guinea Pig IgG1
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with guinea pig IgG1. This may cross react with IgG1 from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:2000-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS containing 0.2% BSA
• Preservative: 0.1% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Goat anti-Guinea Pig IgG1 Secondary Antibody [HRP]

• IgG1
• Igh-4
• RGD1359539
• VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG1 Antibody (NB7389)(3)

Publications using NB7389

• Khattar SK, Samal S, Devico AL et al. Newcastle disease virus expressing human immunodeficiency virus type 1 envelope glycoprotein induces strong mucosal and serum antibody responses in Guinea pigs. J Virol;85(20):10529-10541. 2011-10-01 [PMID: 21849467]
• Hayes BB, Afshari A, Millecchia L et al. Evaluation of percutaneous penetration of natural rubber latex proteins. Toxicol Sci 2000-08-01 [PMID: 10910983]
• Anderson RJ, Pasetti MF, Sztein MB et al. DeltaguaBA attenuated Shigella flexneri 2a strain CVD 1204 as a Shigella vaccine and as a live mucosal delivery system for fragment C of tetanus toxin. Vaccine 2000-04-01 [PMID: 10717338]