Horse anti-Rabbit IgG ImmPRESS™ Secondary Antibody [HRP Polymer]

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7401-NB] - Small bowel: ImmPRESS Anti-Rabbit Ig and DAB (brown) staining using Ki67 primary antibody. Hematoxylin counterstain (blue).

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7401-NB] - Breast Carcinoma: Estrogen receptor (rm), ImmPRESS Anti-Rabbit Ig Kit, DAB (brown) substrate. Hematoxylin QS (blue) counterstain.

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7401-NB] - Breast Carcinoma: Estrogen receptor (rm), ImmPRESS Anti-Rabbit Ig Kit, DAB (brown) substrate. Hematoxylin QS (blue) counterstain.

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7401-NB] - Breast: p53 (rp), ImmPRESS Anti-Rabbit Ig Kit, DAB Substrate Kit (brown). Hematoxylin QS counterstain (blue).

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [HRP Polymer] [MP-7401-NB] - Peyers Patch: ImmPRESS anti-rabbit Ig using Ki67 rabbit primary antibody with Vector NovaRed (red) substrate. Hematoxylin QS counterstain (blue).

• Reactivity: Rb
• Applications: ELISA, Func, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Horse
• Conjugate: HRP Polymer

Horse anti-Rabbit IgG ImmPRESS™ Secondary Antibody [HRP Polymer] Summary

• Description: Features:
• High sensitivity
• Low background
• One-step detection
• Micropolymer technology
• Enhanced accessibility to nuclear and membrane antigens
• Ready-to-use in a convenient dropper bottle
• Shorter assay time
• Simplified multiple labeling
• Immunogen: Rabbit IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Horse
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: No Preservative
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The ImmPRESS(TM) polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step, non-biotin detection system for immunohistochemistry and immunocytochemistry staining. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background.
The peroxidase micropolymers of the ImmPRESS(TM) HRP polymer reagent limit steric interference and provide enhanced accessibility to the target, avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens (such as Ki67, estrogen receptor, bcl-2, CD3, CD8 and CD10) and greater sensitivity than other polymer systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG ImmPRESS(TM) Antibody (MP-7401-NB)(2)

Publications using MP-7401-NB

• Juan Pang, Fitore Raka, Alya Abbas Heirali, Weijuan Shao, Dinghui Liu, Jianqiu Gu, Jia Nuo Feng, Chieko Mineo, Philip W Shaul, Xiaoxian Qian, Bryan Coburn, Khosrow Adeli, Wenhua Ling, Tianru Jin Resveratrol intervention attenuates chylomicron secretion via repressing intestinal FXR-induced expression of scavenger receptor SR-B1. Nature communications 2023-05-11 [PMID: 37160898]
• Pang J, Raka F, Heirali A et al. Dietary resveratrol intervention improves lipid homeostasis via attenuating HFD-induced fecal chenodeoxycholic acid and jejunum SR-B1 elevation Research Square 2022-09-27 (IF/IHC)