Horse anti-Rabbit IgG ImmPRESS™ Secondary Antibody [AP Polymer]

Immunocytochemistry/Immunofluorescence: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue Substrate (blue). Visualized under fluorescence. Broad excitation with peak at 500nm and broad emission with peak at 680nm).

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Colon Cancer: Anti-Ki67 (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue Substrate (blue); Anti-Cox2 (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, ImmPACT Vector Red Substrate (red).

Immunohistochemistry-Paraffin: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, BCIP/NBT Substrate (indigo).

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Colon Cancer: Anti-Ki67 (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue Substrate (blue); Anti-Cox2 (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, ImmPACT Vector Red Substrate (red).

Immunohistochemistry-Paraffin: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue Substrate (blue).

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue Substrate (blue).

Immunohistochemistry: Horse anti-Rabbit IgG ImmPRESS(TM) Secondary Antibody [AP Polymer] [MP-5401-NB] - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, ImmPACT Vector Red Substrate (red).

• Reactivity: Rb
• Applications: ELISA, Func, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Horse
• Conjugate: AP Polymer

Horse anti-Rabbit IgG ImmPRESS™ Secondary Antibody [AP Polymer] Summary

• Description: Features:
• High sensitivity
• Low background
• One-step detection
• Micropolymer technology
• Enhanced accessibility to nuclear and membrane antigens
• Ready-to-use in a convenient dropper bottle
• Shorter assay time
• Simplified multiple labeling
• Immunogen: Rabbit IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Horse
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Preservative: No Preservative
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The ImmPRESS polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The ImmPRESS-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.