Goat anti-Mouse IgG/IgM Secondary Antibody [FITC]

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Flow Cytometry: Goat anti-Human, Mouse IgG/IgM Secondary Antibody [FITC] [NBP1-75213] - human cancer cells stained with mouse antibody and this secondary antibody was used for detection and also as a negative control. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IM
Clonality
Polyclonal
Host
Goat
Conjugate
FITC
Concentration
1.5 mg/ml

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Catalog# & Conjugate Size Price

Goat anti-Mouse IgG/IgM Secondary Antibody [FITC] Summary

Immunogen
Purified Mouse IgG + IgM
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy chains on mouse IgG and heavy chains on mouse IgM and light chains on all mouse immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:20-1:2000
  • Immunomicroscopy 1:20-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NBP1-75213 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
1.5 mg/ml
Purity
>95%, by SDS-PAGE

Notes

Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG/IgM Antibody (NBP1-75213) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-75213:
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Images Ratings Applications Species Date Details
Flow Cytometry IgG/IgM NBP1-75213
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reviewed by:
Ming Lu
Flow Human 07/15/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman cancer cell lines
SpeciesHuman
Lot23-156-032012
CommentsSeveral human cancer cell lines were stained with certain mouse antibody, This Goat anti-Mouse IgG/IgM Antibody [FITC] CAT# NBP1-75213 was used as secondary antibody as well as negative controls.

Blocking

Blocking Details2% FBS in PBS buffer, on ice in dark for 30min

Primary Anitbody

Dilution Rationo primary antibody

Details

Detection Notesthis antibody was used as negative controls
Fixation DetailsCells were detached by Versene, stained on ice in dark for 30min
Permeabilization DetailsNo Permeabilization Steps
Wash Description2% FBS in PBS buffer, wash two times

Comments

CommentsSeveral human cancer cell lines were stained with certain mouse antibody, This Goat anti-Mouse IgG/IgM Antibody [FITC] CAT# NBP1-75213 was used as secondary antibody as well as negative controls.

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Recent Reviews

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Ming Lu
07/15/2014
Application: Flow
Species: Human