Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody

Flow Cytometry: Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody [NBP1-75210] - Human cancer cells stained with mouse antibody and this secondary antibody was used for detection and also as a negative control. Image from verified customer review. Image using the FITC form of this antibody.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, Func, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Goat anti-Mouse IgG/IgM (H+L) Secondary Antibody Summary

• Description: Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
• Immunogen: Purified Mouse IgG + IgM
• Specificity: Based on IEP, this antibody reacts with heavy chains on mouse IgG + IgM and light chains on all mouse immunoglobulins
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunoassay
  • Immunodiffusion
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is suitable for all immunoassay applications.

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin mouse serum proteins

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.05% Sodium Azide
• Purity: Affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG/IgM (H+L) Antibody (NBP1-75210)(1)

Publication using NBP1-75210

• Yu Z, Ju Y, Liu H. Anti-lung cancer effect of glucosamine by suppressing the phosphorylation of FOXO Mol Med Rep 2017-07-18 [PMID: 28713921]