Rabbit anti-Mouse IgG (H+L) Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 646
Format
Pre-adsorbed

Order Details

Rabbit anti-Mouse IgG (H+L) Secondary Antibody [Janelia Fluor 646] (Pre-adsorbed) Summary

Immunogen
Purified Mouse IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins. Minimal cross-reactivity with human IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunocytochemistry 1:10-1:500
  • Immunofluorescence 1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
>95%, by SDS-PAGE

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP2-60654JF646) (0)

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Other Available Formats

Alkaline Phosphatase NBP1-75253
Alkaline Phosphatase NBP1-75252
Allophycocyanin/Cy7 NBP2-60654APCCY7
Biotin NBP2-52988
DyLight 350 NBP2-60654UV
DyLight 405 NBP2-60654V
DyLight 488 NBP2-60654G
DyLight 550 NBP1-76218
DyLight 594 NBP2-60654DL594
DyLight 650 NBP2-60654C
DyLight 680 NBP2-60654FR
DyLight 755 NBP2-60654IR
FITC NBP1-75254
HRP NBP2-52989
Janelia Fluor 549 NBP2-60654JF549
Janelia Fluor 646 NBP2-60654JF646
PE/Atto594 NBP2-60654PEATT594
PE/Cy5.5 NBP2-60654PECY55
PE/Cy7 NBP2-60654PECY7

Additional IgG (H+L) Products

Array NBP2-60654JF646

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