Rabbit anti-Mouse IgG (H+L) Secondary Antibody [FITC] (Pre-absorbed)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC
Format
Pre-absorbed

Order Details

Rabbit anti-Mouse IgG (H+L) Secondary Antibody [FITC] (Pre-absorbed) Summary

Immunogen
Purified Mouse IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human IgG serum proteins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:20000
  • ELISA 1:2000 - 1:20000
  • Immunohistochemistry 1:500 - 1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-75254 in the following applications:

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Purity > 95% based on SDS-PAGE. Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Review for IgG (H+L) Antibody (NBP1-75254) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-75254:
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Images Ratings Applications Species Date Details
Flow Cytometry IgG (H+L) NBP1-75254
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reviewed by:
Anonymous
Flow Human 11/30/2017
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Summary

ApplicationFlow Cytometry
Sample Testedcancer cell line
SpeciesHuman
Lot24-81-052213

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Anonymous
11/30/2017
Application: Flow
Species: Human