Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed)

Images

 
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - DyLight 488 conjugated anti-Rabbit IgG was used to demonstrate 2 color STED immunofluorescence ...read more
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at ...read more
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB7575] - Lane 1: Rabbit IgG. Load: 50 ng per lane. ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Image from the DyLight 488 version of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Using the DyLight 405 format of this antibody.
SDS-Page: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - SDS-Page of Goat anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed).Lane 1: Goat anti-Rabbit IgG - ...read more
Western Blot of Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed)

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, PAGE
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Format
Pre-adsorbed

Order Details

Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) Summary

Description
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
Immunogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with rabbit whole IgG molecule in goat.
Specificity
This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat, and Sheep Serum Proteins.

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA against at less than 1% of target signal.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:500 - 1:2000
Application Notes
This secondary antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Publications
Read Publications using NBP1-72732.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-72732)(16)

We have publications tested in 6 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl, IHC-P, WB.


Filter By Application
ICC/IF
(6)
IF/IHC
(2)
IHC-Fr
(1)
IHC-FrFl
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP1-72732 Applications Species
Yang Y, Chen C, Zuo Q Et al. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification Sci Adv 2022-12-09 [PMID: 36490339] (ICC/IF) ICC/IF
Atzori MG, Ceci C, Ruffini F Et al. The Anti-Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1) D16F7 Monoclonal Antibody Inhibits Melanoma Adhesion to Soluble VEGFR-1 and Tissue Invasion in Response to Placenta Growth Factor Cancers (Basel) 2022-11-26 [PMID: 36428669] (IHC-P)

Details:
Citation using the HRP version of this antibody.
IHC-P
Certel SJ, McCabe BD, Stowers RS. Et al. A conditional GABAergic synaptic vesicle marker for Drosophila J Neurosci Methods 2022-02-27 [PMID: 35219770] (IF/IHC)

Details:
Citation using the Janelia Fluor 549 version of this antibody.
IF/IHC
Arguello T, Peralta S, Antonicka H et al. ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly Cell reports 2021-12-21 [PMID: 34936866]
Brady J, Vasin A, Bykhovskaia M. Et al. The Accessory Helix of Complexin Stabilizes a Partially Unzippered State of the SNARE Complex and Mediates the Complexin Clamping Function In Vivo eNeuro 2021-03-11 [PMID: 33692090] (IF/IHC)

Details:
Citation using the Cy3 version of this antibody.
IF/IHC
Wilson RH, Carney PR, Glover E, et al. Generation of an Allelic Series at the Ahr Locus Using an Edited Recombinant Approach Toxicol Sci 2021-01-22 [PMID: 33480436] (WB) WB
Liu JT, Balmori-Cedeno J, Misk E, Lumsden JS Pharmacological and nutritional modulation of autophagy in a rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1 In Vitro Cell Dev Biol Anim 2020-09-09 [PMID: 32901427] (ICC/IF) ICC/IF
Zhou S, Wang R, Xiao H Adipocytes induce the resistance of ovarian cancer to carboplatin through ANGPTL4 Oncol Rep 2020-06-16 [PMID: 32705217] (ICC/IF)

Details:
Citation using the Rhodamine format of this antibody.
ICC/IF
McKinney H. M, Sherer L. M, et al. Characterization of Drosophila octopamine receptor neuronal expression using MiMIC-converted Gal4 lines. J Comp Neurol 2020-09-01 [PMID: 32060912]

Details:
Citation using the Janiela Fluor 549 format of this antibody.
Tison K. V, McKinney H. M, et al. Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using Drosophila vAChT. G3 (Bethesda) 2020-02-06 [PMID: 31767639] (ICC/IF) ICC/IF
Show All 16 Publications.

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