Goat anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed)

Images

 
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Myosin expression in HeLa cells. Cells were fixed in 4% paraformaldehyde for 20 miniutes at room ...read more
Flow Cytometry: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG, H/L Chains Antibody [DyLight 405] [NBP1-72957]
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Lane 1: Rabbit IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for ...read more
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB7575] - Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng ...read more
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Texas Red] [NB120-6719] - Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Image from the DyLight 488 version of this antibody.
Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - DyLight 488 conjugated anti-Rabbit IgG was used to demonstrate 2 color STED immunofluorescence ...read more
SDS-Page: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Lane 1: Goat anti-Rabbit IgG-Non-Reduced. Lane 2: Goat anti-Rabbit IgG-Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 25 & ...read more
Dot Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Dot Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB7575] - Rabbit IgG. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane ...read more
Dot Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Dot Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Texas Red] [NB120-6719] - A three-fold serial dilution of Rabbit IgG ...read more
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Goat anti-Rabbit IgG, H/L Chains Antibody [DyLight 800] [NBP1-72954]
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP1-72732] - Fluorophore-linked immunosorbent assay: Rabbit IgG, H/L Chains Antibody [DyLight 488] [NBP1-72944] ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, DB, ELISA, FLISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, PAGE
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Format
Pre-adsorbed
Concentration
1 mg/ml

Goat anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) Summary

Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:15000 - 1:30000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Frozen 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
Application Notes
This product is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Publications
Read Publication using NBP1-72732.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-72732)(1)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional IgG (H+L) Products

IgG (H+L) NBP1-72732

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