Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed)

Images

 
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - DyLight 488 conjugated anti-Rabbit IgG was used to demonstrate 2 color STED immunofluorescence ...read more
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Lane 1: Rabbit IgG. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1,000 for overnight at ...read more
Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB7575] - Lane 1: Rabbit IgG. Load: 50 ng per lane. ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Image from the DyLight 488 version of this antibody.
Fluorophore-linked immunosorbent assay: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - Using the DyLight 405 format of this antibody.
SDS-Page: Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) [NBP1-72732] - SDS-Page of Goat anti-Rabbit IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed).Lane 1: Goat anti-Rabbit IgG - ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, FLISA, ICC/IF, IHC, PAGE
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Format
Pre-adsorbed

Order Details

Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) Summary

Immunogen
Goat anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with rabbit whole IgG molecule in goat.
Specificity
This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat, and Sheep Serum Proteins.

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA against at less than 1% of target signal.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:500 - 1:2000
Application Notes
This secondary antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Publications
Read Publications using
NBP1-72732 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-72732)(8)

We have publications tested in 1 confirmed species: Drosophila melanogaster.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(4)
WB
(1)
All Applications
Filter By Species
Drosophila melanogaster
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-72732 Applications Species
Arguello T, Peralta S, Antonicka H et al. ATAD3A has a scaffolding role regulating mitochondria inner membrane structure and protein assembly Cell reports Dec 21 2021 [PMID: 34936866]
Wilson RH, Carney PR, Glover E, et al. Generation of an Allelic Series at the Ahr Locus Using an Edited Recombinant Approach Toxicol Sci 2021 Jan 22 [PMID: 33480436] (WB) WB
Liu JT, Balmori-Cedeno J, Misk E, Lumsden JS Pharmacological and nutritional modulation of autophagy in a rainbow trout (Oncorhynchus mykiss) gill cell line, RTgill-W1 In Vitro Cell Dev Biol Anim 2020 Sep 09 [PMID: 32901427] (ICC/IF) ICC/IF
Zhou S, Wang R, Xiao H Adipocytes induce the resistance of ovarian cancer to carboplatin through ANGPTL4 Oncol Rep Jun 16 2020 [PMID: 32705217] (ICC/IF)

Details:
Citation using the Rhodamine format of this antibody.
ICC/IF
McKinney HM, Sherer LM, Williams JL et al. Characterization of drosophila octopamine receptor neuronal expression using MiMIC-converted Gal4 lines J. Comp. Neurol. Feb 14 2020 [PMID: 32060912]
Tison K. V, McKinney H. M, et al. Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection Using Drosophila vAChT. G3 (Bethesda) 2020-02-06 [PMID: 31767639] (ICC/IF, Drosophila melanogaster)

Details:
Citation using the Janiela Fluor 549 format of this antibody.
ICC/IF Drosophila melanogaster
Posteraro B, Paroni Sterbini F, Petito V et al. Liver Injury, Endotoxemia, and Their Relationship to Intestinal Microbiota Composition in Alcohol-Preferring Rats Alcohol Clin Exp Res Oct 16 2018 [PMID: 30320890] (ICC/IF) ICC/IF
Chen LL, Chen X, Choi H et al. Exploiting antitumor immunity to overcome relapse and improve remission duration. Cancer Immunol Immunother. [PMID: 22198309]

Details:
Citation using the Texas Red form of this antibody.

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