Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed)

Images

 
Western Blot: Goat anti-Rabbit IgG Secondary Antibody [Cy3] [NB120-6939] - Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Cy3 Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Cy3
Format
Pre-adsorbed

Order Details

Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed) Summary

Immunogen
This Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed) was developed against rabbit IgG whole molecule.
Specificity
Assay of Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed) by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum, Rabbit IgG and Rabbit serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep serum proteins. This antibody was pre-adsorbed against bovine chicken goat guinea pig hamster horse human mouse rat & sheep serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot
Application Notes
Use in IHC reported in scientific literature (PMID:33692090). Use in Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating was reported in the scientific literature (PMID: 25446008).
Publications
Read Publications using NB120-6939.

Packaging, Storage & Formulations

Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB120-6939)(6)

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl.


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ICC/IF
(1)
IF/IHC
(1)
IHC-Fr
(1)
IHC-FrFl
(1)
All Applications
Filter By Species
All Species
Showing Publications 1 - 6 of 6.
Publications using NB120-6939 Applications Species
Oliva Cárdenas A, Zamora-Rodríguez BC, Batalla-García KA et al. Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats Journal of visualized experiments : JoVE 2023-04-07 [PMID: 37092839]
Brady J, Vasin A, Bykhovskaia M. Et al. The Accessory Helix of Complexin Stabilizes a Partially Unzippered State of the SNARE Complex and Mediates the Complexin Clamping Function In Vivo eNeuro 2021-03-11 [PMID: 33692090] (IF/IHC)

Details:
Citation using the Cy3 version of this antibody.
IF/IHC
Zhu G, Wang J, Song M Et al. Overexpression of Jagged1 Ameliorates Aged Rat-Derived Endothelial Progenitor Cell Functions and Improves Its Transfusion Efficiency for Rat Balloon-Induced Arterial Injury Ann Vasc Surg 2017-02-07 [PMID: 28163178]

Details:
Citation using the Cy3 version of this antibody.
Garafola CS, Henn FA. A change in hippocampal protocadherin gamma expression in a learned helpless rat Brain Res 2014-12-03 [PMID: 25446008] (IHC-FrFl, IHC-Fr)

Details:
Citation using the Cy3 version of this antibody.
IHC-FrFl, IHC-Fr
Wang T, Gilkes DM, Takano N Et al. Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis Proc Natl Acad Sci U S A 2014-06-19 [PMID: 24938788] (ICC/IF)

Details:
Citation using the Cy3 version of this antibody.
ICC/IF
Rowland MM, Gong D, Bostic HE Et al. Microarray analysis of Akt PH domain binding employing synthetic biotinylated analogs of all seven phosphoinositide headgroup isomers Chem Phys Lipids 2011-12-20 [PMID: 22178158]

Details:
Citation using the Cy3 version of this antibody.

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Video Protocols

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