Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed)

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Western Blot: Goat anti-Rabbit IgG Secondary Antibody [Cy3] [NB120-6939] - Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Cy3 Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Cy3
Format
Pre-adsorbed

Order Details

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed) Summary

Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
Publications
Read Publications using NB120-6939.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Background

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB120-6939)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB120-6939) (0)

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Additional Array Products

Array NB120-6939

Bioinformatics Tool for IgG (H+L) Antibody (NB120-6939)

Discover related pathways, diseases and genes to IgG (H+L) Antibody (NB120-6939). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on IgG (H+L)

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Calculators

Concentration Calculator

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Bioinformatics

Gene Symbol IGHG