Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed)


Western Blot: Goat anti-Rabbit IgG Secondary Antibody [Cy3] [NB120-6939] - Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Cy3 Conjugated Secondary Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per ...read more

Product Details

Reactivity RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC

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Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed) Summary

This Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed) was developed against rabbit IgG whole molecule.
Assay of Goat anti-Rabbit IgG (H+L) Secondary Antibody [Cy3] (Pre-adsorbed) by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum, Rabbit IgG and Rabbit serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep serum proteins. This antibody was pre-adsorbed against bovine chicken goat guinea pig hamster horse human mouse rat & sheep serum proteins.
Immunogen affinity purified


  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot
Application Notes
Use in IHC reported in scientific literature (PMID:33692090). Use in Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating was reported in the scientific literature (PMID: 25446008).
Read Publications using NB120-6939.

Packaging, Storage & Formulations

Store at -20C in the dark. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01% Sodium Azide
Immunogen affinity purified


Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).


1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB120-6939)(6)

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-FrFl.

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Showing Publications 1 - 6 of 6.
Publications using NB120-6939 Applications Species
Oliva Cárdenas A, Zamora-Rodríguez BC, Batalla-García KA et al. Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats Journal of visualized experiments : JoVE 2023-04-07 [PMID: 37092839]
Brady J, Vasin A, Bykhovskaia M. Et al. The Accessory Helix of Complexin Stabilizes a Partially Unzippered State of the SNARE Complex and Mediates the Complexin Clamping Function In Vivo eNeuro 2021-03-11 [PMID: 33692090] (IF/IHC)

Citation using the Cy3 version of this antibody.
Zhu G, Wang J, Song M Et al. Overexpression of Jagged1 Ameliorates Aged Rat-Derived Endothelial Progenitor Cell Functions and Improves Its Transfusion Efficiency for Rat Balloon-Induced Arterial Injury Ann Vasc Surg 2017-02-07 [PMID: 28163178]

Citation using the Cy3 version of this antibody.
Garafola CS, Henn FA. A change in hippocampal protocadherin gamma expression in a learned helpless rat Brain Res 2014-12-03 [PMID: 25446008] (IHC-FrFl, IHC-Fr)

Citation using the Cy3 version of this antibody.
Wang T, Gilkes DM, Takano N Et al. Hypoxia-inducible factors and RAB22A mediate formation of microvesicles that stimulate breast cancer invasion and metastasis Proc Natl Acad Sci U S A 2014-06-19 [PMID: 24938788] (ICC/IF)

Citation using the Cy3 version of this antibody.
Rowland MM, Gong D, Bostic HE Et al. Microarray analysis of Akt PH domain binding employing synthetic biotinylated analogs of all seven phosphoinositide headgroup isomers Chem Phys Lipids 2011-12-20 [PMID: 22178158]

Citation using the Cy3 version of this antibody.

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