Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)

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Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7570] - analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by goat anti-mouse IgG HRP as a secondary antibody. Image ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Format
Pre-absorbed
Concentration
0.5 mg/ml

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Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Mouse IgG-heavy and light chain
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig and rabbit IgG was detected. This antibody may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:50000
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
  • Immunohistochemistry-Paraffin 1:200 - 1:5000
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Reviewed Applications
Read 1 Review rated 5
using
NB7570 in the following applications:

Publications
Read Publications using
NB7570 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.1% Pro-Clean 400
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7570)(3)

Review for IgG (H+L) Antibody (NB7570) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB7570:
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WB
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NB7570
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reviewed by:
Anonymous
WB Human 10/07/2014
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Summary

ApplicationWestern Blot
Sample TestedSample tested: Jurkat cells
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBST

Primary Anitbody

Dilution Ratio1:1K in PBST buffer

Secondary Antibody

Secondary DescriptionGoat anti-Mouse IgG Antibody (HRP)-Novus
Secondary Manufacturer Cat#Cat No NB7570
Secondary ConcentrationDiluted 5,000 times

Details

Detection NotesDetected using PromoKine ECL Chemiluminescence Detection Kit with a 1 min exposure on X-ray film

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7570) (0)

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Anonymous
10/07/2014
Application: WB
Species: Human