Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed)


Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7567] - Analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by HRP conjugated Goat anti-Mouse IgG (H+L) ...read more

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Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
0.5 mg/ml

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Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) Summary

This Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against mouse IgG .
By immunoelectrophoresis and ELISA this Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, chicken, horse, human, pig and rabbit immunosorbents to remove cross reactive Antibodies. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig and rabbit IgG was detected. This antibody may cross react with IgG from other species.
Immunogen affinity purified


  • Immunocytochemistry/ Immunofluorescence 1:100- 1:1000
  • Immunohistochemistry 1:100- 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:5000
Read Publications using NB7567.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Phosphate Buffered Saline (PBS)
0.09% Sodium Azide
0.5 mg/ml
Immunogen affinity purified


Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).


1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7567)(11)

We have publications tested in 3 applications: ELISA, IA, WB.

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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB7567 Applications Species
Del Rosario Garcia-Lozano M, Dragoni F, Gallego P et al. Piperazine-derived small molecules as potential Flaviviridae NS3 protease inhibitors. In vitro antiviral activity evaluation against Zika and Dengue viruses Bioorganic chemistry 2023-02-04 [PMID: 36801791] (IA) IA
S Padmanabha, B Gaire, Y Zou, MM Uddin, I Vancurova IFNgamma-induced PD-L1 expression in ovarian cancer cells is regulated by JAK1, STAT1 and IRF1 signaling Cellular Signalling, 2022-07-09;97(0):110400. 2022-07-09 [PMID: 35820543] (WB) WB
Abd El-Lateef HM, El-Dabea T, Khalaf MM Et al. Development of Metal Complexes for Treatment of Coronaviruses Int J Mol Sci 2022-06-24 [PMID: 35742870] (IA)

Citation using the HRP version of this antibody.
Cirri D, Marzo T, Tolbatov I Et al. In Vitro Anti-SARS-CoV-2 Activity of Selected Metal Compounds and Potential Molecular Basis for Their Actions Based on Computational Study Biomolecules 2021-12-24 [PMID: 34944502] (IA)

Citation using the HRP version of this antibody.
Vicenti I, Martina MG, Boccuto A et al. System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2 Eur J Med Chem 2021-07-17 [PMID: 34273661] (ELISA)

This citation used the HRP format of this antibody.
Boccuto A, Dragoni F, Picarazzi F et al. Sofosbuvir Selects for Drug-Resistant Amino Acid Variants in the Zika Virus RNA-Dependent RNA-Polymerase Complex In Vitro Int J Mol Sci 2021-04-03 [PMID: 33800884] (WB) WB
Riva V, Garbelli A, Brai A et al. Unique Domain for a Unique Target: Selective Inhibitors of Host Cell DDX3X to Fight Emerging Viruses J. Med. Chem. 2020-08-18 [PMID: 32787106]

Citation using the HRP format of this antibody.
Dragoni F, Boccuto A, Picarazzi F et al. Evaluation of sofosbuvir activity and resistance profile against West Nile virus in vitro Antiviral Res 2020-01-14 [PMID: 31931104] (ELISA) ELISA
Wu L, Zhang Z, Pan X et al. Expression and contribution of the HIF-1 alpha/VEGF signaling pathway to luteal development and function in pregnant rats Mol Med Rep 2015-09-02 [PMID: 26323652]
Pang X, Wang Z, Yin D, Zhang Z. Overexpression of hypoxia-inducible factor prolyl- hydoxylase attenuated by HCG-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep 2015-05-16 [PMID: 25975603]
Show All 11 Publications.

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