Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed)

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Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) [NB7567] - Analysis of Jurkat cell lysate using anti-MBD1 antibody (Cat. # NB100-56537) followed by HRP conjugated Goat anti-Mouse IgG (H+L) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Format
Pre-adsorbed
Concentration
0.5 mg/ml

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Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) Summary

Immunogen
This Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against mouse IgG .
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, chicken, horse, human, pig and rabbit immunosorbents to remove cross reactive Antibodies. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, pig and rabbit IgG was detected. This antibody may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:100- 1:1000
  • Immunohistochemistry 1:100- 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:5000
Publications
Read Publications using
NB7567 in the following applications:

  • 2 publications
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7567)(7)

We have publications tested in 1 confirmed species: Monkey.

We have publications tested in 2 applications: ELISA, WB.


Filter By Application
ELISA
(2)
WB
(2)
All Applications
Filter By Species
Monkey
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB7567 Applications Species
Vicenti I, Martina MG, Boccuto A et al. System-oriented optimization of multi-target 2,6-diaminopurine derivatives: Easily accessible broad-spectrum antivirals active against flaviviruses, influenza virus and SARS-CoV-2 Eur J Med Chem Jul 17 2021 [PMID: 34273661] (ELISA)

Details:
This citation used the HRP format of this antibody.
ELISA
Boccuto A, Dragoni F, Picarazzi F et al. Sofosbuvir Selects for Drug-Resistant Amino Acid Variants in the Zika Virus RNA-Dependent RNA-Polymerase Complex In Vitro Int J Mol Sci Apr 3 2021 [PMID: 33800884] (WB, Monkey)

Details:
Citation using the HRP format of this antibody.
WB Monkey
Riva V, Garbelli A, Brai A et al. Unique Domain for a Unique Target: Selective Inhibitors of Host Cell DDX3X to Fight Emerging Viruses J. Med. Chem. Aug 18 2020 [PMID: 32787106]

Details:
Citation using the HRP format of this antibody.
Dragoni F, Boccuto A, Picarazzi F et al. Evaluation of sofosbuvir activity and resistance profile against West Nile virus in vitro Antiviral Res Jan 14 2020 [PMID: 31931104] (ELISA) ELISA
Wu L, Zhang Z, Pan X et al. Expression and contribution of the HIF-1 alpha/VEGF signaling pathway to luteal development and function in pregnant rats Mol Med Rep Sep 2 2015 [PMID: 26323652]
Zhang Z, Pang X, Tang Z et al. Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells. Mol Med Rep. 2015 Sep [PMID: 25975603]

Details:
This citation used the HRP form of this antibody.
Kang ML, Kwon JS, Kim MS. Induction of neuronal differentiation of rat muscle-derived stem cells in vitro using basic fibroblast growth factor and ethosuximide Int J Mol Sci Mar 27 2013 [PMID: 23528890] (WB) WB

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