Goat anti-Mouse IgG (H+L) Secondary Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by goat anti-mouse IgG FITC ...read more
Flow Cytometry: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by goat anti-mouse IgG FITC conjugated secondary ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

Goat anti-Mouse IgG (H+L) Secondary Antibody Summary

Immunogen
Mouse IgG-heavy and light chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000- 1:30000
  • ELISA 1:1000-1:30000 For Coating-1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
Publications
Read Publications using
NB7535 in the following applications:

  • 1 publication
  • 2 publications
  • IHC
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7535)(13)

We have publications tested in 2 confirmed species: Mouse, Parasite.

We have publications tested in 4 applications: Flow, ICC/IF, IHC, WB.


Filter By Application
Flow
(1)
ICC/IF
(2)
IHC
(1)
WB
(3)
All Applications
Filter By Species
Mouse
(3)
Parasite
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB7535 Applications Species
Ebneter JA, Heusser SD, Schraner EM et al. Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia. Nat Commun. [PMID: 27976675] (WB, Parasite)

Details:
Citation using the HRP form of this antibody.
WB Parasite
Kwon TR, Oh CT, Choi EJ et al. Human placental extract exerts hair growth-promoting effects through the GSK-3b signaling pathway in human dermal papilla cells. Int J Mol Med. [PMID: 26311045] (ICC/IF)

Details:
Citation using the FITC form of this antibody.
ICC/IF
Moghimi E, Solomon JA, Gianforcaro A, Hamadeh MJ. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One. [PMID: 26020962] (WB, Mouse)

Details:
Citation using the HRP form of this antibody.
WB Mouse
Meerson A, Traurig M, Ossowski V et al. Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia. [PMID: 23756832] (WB, Mouse)

Details:
Citation using the HRP form of this antibody.
WB Mouse
Yoon J, Juhn KM, Ko JK et al. Effects of oxygen tension and IGF-I on HIF-1alpha protein expression in mouse blastocysts. J Assist Reprod Genet. [PMID: 23232974] (IHC, ICC/IF, Mouse)

Details:
Citation using the FITC form of this antibody.
IHC, ICC/IF Mouse
Giessrigl B, Krieger S, Rosner M et al. Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells. Hum Mol Genet. [PMID: 22843495]

Details:
Citation using the HRP form of this antibody.
Lin YT, Ding JY, Li MY et al. YAP regulates neuronal differentiation through Sonic hedgehog signaling pathway. Exp Cell Res. [PMID: 22659622]

Details:
Citation using the HRP form of this antibody.
Kim HJ, Lim SJ, Kwag HL et al. The choice of resin-bound ligand affects the structure and immunogenicity of column-purified human papillomavirus type 16 virus-like particles. PLoS One. 2012 [PMID: 22563414]

Details:
Citation using the HRP form of this antibody.
Wilkerson et al. Lineage differentiation of canine lymphoma/leukemias and aberrant expression of CD molecules. Vet Immunol Immunopathol. [PMID: 15963817] (Flow)

Details:
Citation using the FITC form of this antibody.
Flow
Soenawan E, Srivastava I, Gupta S et al. Maintenance of long-term immunological memory by low avidity IgM-secreting cells in bone marrow after mucosal immunizations with cholera toxin adjuvant. Vaccine. [PMID: 15063581]

Details:
Citation using the HRP form of this antibody.
Show All 13 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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