Goat anti-Mouse IgG (H+L) Secondary Antibody

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Western Blot: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Image using the HRP format of Goat anti-Mouse IgG (H+L) Secondary Antibody.
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by Goat anti-Mouse IgG (H+L) ...read more
Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. ...read more
Flow Cytometry: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by goat anti-mouse IgG FITC conjugated secondary ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Catalog# & Conjugate Size Price

Goat anti-Mouse IgG (H+L) Secondary Antibody Summary

Immunogen
This Goat anti-Mouse IgG (H+L) Secondary Antibody was developed against mouse IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Mouse IgG (H+L) Secondary Antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000- 1:30000
  • ELISA 1:1000-1:30000 For Coating-1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
Publications
Read Publications using
NB7535 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7535)(27)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 6 applications: FLOW, Flow, ICC/IF, IHC, IHC-P, WB.


Filter By Application
FLOW
(1)
Flow
(1)
ICC/IF
(5)
IHC
(3)
IHC-P
(1)
WB
(8)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB7535 Applications Species
Meitei HY, Uppangala S, Sharan K et al. A Simple, Centrifugation-Free, Sperm-Sorting Device Eliminates the Risks of Centrifugation in the Swim-Up Method While Maintaining Functional Competence and DNA Integrity of Selected Spermatozoa Reprod Sci Jul 30 2020 [PMID: 32734563] (ICC/IF, Human)

Details:
Citation using the FITC format of this antibody.
ICC/IF Human
Ismail A, Doghish AS, E M Elsadek B et al. Hydroxycitric acid potentiates the cytotoxic effect of tamoxifen in MCF-7 breast cancer cells through inhibition of ATP citrate lyase Steroids May 23 2020 [PMID: 32439410] (WB)

Details:
Citation using the Alkaline Phosphatase format of this antibody.
WB
Han Y, Sanford L, Simpson DM et al. Remodeling of Zn(2+) homeostasis upon differentiation of mammary epithelial cells Metallomics Jan 18 2020 [PMID: 31950952] (WB) WB
Katsumi T, Guicciardi ME, Azad A et al Activated cholangiocytes release macrophage-polarizing extracellular vesicles bearing the DAMP S100A11. Am J Physiol Cell Physiol. 2019 Oct 1 [PMID: 31365294]

Details:
Citation using the HRP version of this antibody.
Janssen L, Saranya M, Leinonen M et al. Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation Carbon 2019 Nov 1 (IHC, Human)

Details:
Citation using the FITC format of this antibody.
IHC Human
Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol Sep 26 2019 [PMID: 31551961] (WB) WB
Elsadek BEM, Abdelghany AA, Abd El-Aziz MA et al Validation of the Diagnostic and Prognostic Values of ADAMTS5 and FSTL1 in Osteoarthritis Rat Model Cartilage. 2019 Jun 10 [PMID: 31177809]

Details:
Citation using the Alkaline Phosphatase version of this antibody.
Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep Feb 7 2019 [PMID: 30723259] (IHC-P) IHC-P
Lu J, Shen X, Sun X et al. Increased recruitment of endogenous stem cells and chondrogenic differentiation by a composite scaffold containing bone marrow homing peptide for cartilage regeneration Theranostics Nov 16 2018 [PMID: 30429885] (IHC) IHC
Kwon TR, Oh CT, Bak DH et al. Effects on skin of Stichopus japonicus viscera extracts detected with saponin including Holothurin A: Down-regulation of melanin synthesis and up-regulation of neocollagenesis mediated by ERK signaling pathway J Ethnopharmacol Aug 14 2018 [PMID: 30102992] (ICC/IF) ICC/IF
Show All 27 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7535) (0)

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Other Available Formats

Alkaline Phosphatase NB7536
Biotin NB7537
FITC NB7538
HRP NB7539

Additional IgG (H+L) Products

Array NB7535

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