Goat anti-Mouse IgG (H+L) Secondary Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by goat anti-mouse IgG FITC ...read more
Flow Cytometry: Goat anti-Mouse IgG (H+L) Secondary Antibody [NB7535] - Detection of RYK in human primary colon cancer cell line using anti-RYK antibody followed by goat anti-mouse IgG FITC conjugated secondary ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

Goat anti-Mouse IgG (H+L) Secondary Antibody Summary

Immunogen
Mouse IgG-heavy and light chain
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000- 1:30000
  • ELISA 1:1000-1:30000 For Coating-1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
Publications
Read Publications using
NB7535 in the following applications:

  • 1 publication
  • 1 publication
  • 3 publications
  • IHC
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7535)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Parasite.

We have publications tested in 5 applications: FLOW, Flow, ICC/IF, IHC, WB.


Filter By Application
FLOW
(1)
Flow
(1)
ICC/IF
(3)
IHC
(1)
WB
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(6)
Parasite
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB7535 Applications Species
Wang X, Hybiske K, Stephens RS. Direct visualization of the expression and localization of chlamydial effector proteins within infected host cells. Pathog Dis. 2018 Jan 30 [PMID: 29390129] (WB, Mouse)

Details:
This citation used the HRP form of this antbody
WB Mouse
Perlenfein TJ, Murphy RM. A mechanistic model to predict effects of cathepsin B and cystatin C on b-amyloid aggregation and degradation. J Biol Chem. 2017 Oct 18 [PMID: 29046353] (WB, Mouse)

Details:
This citation used the HRP form of this antibody.
WB Mouse
Saribas AS, DeVoto J, Golla A et al. Discovery and Characterization of Novel Trans-Spliced Products of Human Polyoma JC Virus Late Transcripts from PML Patients. J Cell Physiol. 2017 Oct 16 [PMID: 29044559] (Mouse)

Details:
This citation used the FITC form of this antibody.
Mouse
England C, Jiang D, Hernandez R et al. ImmunoPET Imaging of CD146 in Murine Models of Intrapulmonary Metastasis of Non-Small Cell Lung Cancer. Mol Pharm 2017 Sep 06 [PMID: 28825843] (ICC/IF, FLOW, Human) ICC/IF, FLOW Human
Ebneter JA, Heusser SD, Schraner EM et al. Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia. Nat Commun. [PMID: 27976675] (WB, Parasite)

Details:
Citation using the HRP form of this antibody.
WB Parasite
Kwon TR, Oh CT, Choi EJ et al. Human placental extract exerts hair growth-promoting effects through the GSK-3b signaling pathway in human dermal papilla cells. Int J Mol Med. [PMID: 26311045] (ICC/IF)

Details:
Citation using the FITC form of this antibody.
ICC/IF
Moghimi E, Solomon JA, Gianforcaro A, Hamadeh MJ. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One. [PMID: 26020962] (WB, Mouse)

Details:
Citation using the HRP form of this antibody.
WB Mouse
Meerson A, Traurig M, Ossowski V et al. Human adipose microRNA-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia. [PMID: 23756832] (WB, Mouse)

Details:
Citation using the HRP form of this antibody.
WB Mouse
Yoon J, Juhn KM, Ko JK et al. Effects of oxygen tension and IGF-I on HIF-1alpha protein expression in mouse blastocysts. J Assist Reprod Genet. [PMID: 23232974] (IHC, ICC/IF, Mouse)

Details:
Citation using the FITC form of this antibody.
IHC, ICC/IF Mouse
Giessrigl B, Krieger S, Rosner M et al. Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells. Hum Mol Genet. [PMID: 22843495]

Details:
Citation using the HRP form of this antibody.
Show All 17 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional IgG (H+L) Products

IgG (H+L) NB7535

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