By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.
2% cold fish geletin in TBS buffer, room temperature for 1hour
1:3000 overnight at 4C in blocking buffer
Rabbit IgG Antibody (NB7185)
Secondary Manufacturer Cat#
15-second exposure shows strong band at correct MW.
This antibody claimed it has Less than 0.1% cross reactivity to mouse and human IgG, which is the reason I purchase this antibody. In my experience, it did not bind to any mouse IgG. It binds perfectly with rabbit IgG.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.