Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed)

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP
Format
Pre-adsorbed
Concentration
0.5 mg/ml

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Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) Summary

Immunogen
This Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was developed against rabbit IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive Antibodies. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected. This antibody may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
  • Immunohistochemistry-Paraffin 1:200 - 1:5000
  • Western Blot 1:5000 - 1:50000
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7185 in the following applications:

Publications
Read Publications using NB7185.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NB7185)(3)

We have publications tested in 2 applications: IHC, WB.


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IHC
(1)
WB
(1)
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Showing Publications 1 - 3 of 3.
Publications using NB7185 Applications Species
Chae M, Son ED, Bae IH et al. UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes Exp Mol Med Feb 22 2020 [PMID: 32080341] (IHC) IHC
Dovmark TH, Saccomano M, Hulikova A et al. Connexin-43 channels are a pathway for discharging lactate from glycolytic pancreatic ductal adenocarcinoma cells Oncogene Apr 4 2017 [PMID: 28368405] (WB) WB
Cimenci C, Blackburn N, Sedlakova V Et al. Combined Methylglyoxal Scavenger and Collagen Hydrogel Therapy Prevents Adverse Remodeling and Improves Cardiac Function Post Myocardial Infarction Advanced Functional Materials Oct 12 2021

Review for IgG (H+L) Antibody (NB7185) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB7185:
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Images Ratings Applications Species Date Details
Western Blot IgG (H+L) NB7185
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5
reviewed by:
Verified Customer
WB Other 05/22/2015
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Summary

ApplicationWestern Blot
Sample Testedcos7 whole cell lysate
SpeciesOther
LotA11

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Ratio1:3000 overnight at 4C in blocking buffer

Secondary Antibody

Secondary DescriptionRabbit IgG Antibody (NB7185)
Secondary Manufacturer Cat#NB7185
Secondary Concentration1:5000

Details

Detection Notes15-second exposure shows strong band at correct MW.

Comments

CommentsThis antibody claimed it has Less than 0.1% cross reactivity to mouse and human IgG, which is the reason I purchase this antibody. In my experience, it did not bind to any mouse IgG. It binds perfectly with rabbit IgG.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NB7185) (0)

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Other Available Formats

HRP NB7185

Additional IgG (H+L) Products

Array NB7185

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Contact Information

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Recent Reviews

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Verified Customer
05/22/2015
Application: WB
Species: Other