Goat IgG Fc Isotype Control

SDS-Page: Goat IgG Fc Isotype Control [NBP1-97042] - SDS-PAGE of Goat IgG F(c) Fragment. Lane M: 5 uL Opal Prestained Marker. Lane 1: Reduced Goat IgG Whole Molecule. Lane 2: Reduced Goat IgG F(c) Fragment. Lane 3: Reduced Goat IgG F(ab) Fragment. Lane 4: Reduced Goat IgM Whole Molecule. Load: 1 ug for IgG, F(c) and F(ab); 3 ug for IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.

Dot Blot: Goat IgG Fc Isotype Control [NBP1-97042] - Dot Blot: Goat IgG Fc Isotype Control [Rhodamine] [NBP1-96842] - Dot Blot of Rhodamine Conjugated Goat IgG Fc. Dotted Rhodamine Conjugated Goat IgG Fc with following concentrations. Load: Lane 1 - 50ng Lane 2 - 16.67ng Lane 3 - 5.56ng Lane 4 - 1.85ng Lane 5 - 0.62ng Image using the Rhodamine form of this antibody.

Product Discontinued

Goat IgG Fc Isotype Control Summary

• Description: Native Goat IgG, Fc.

  Source: Serum

• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Goat IgG and anti-Goat IgG F(c). No reaction was observed against anti-Goat IgG F(ab)2 or anti-Papain.
• Isotype: IgG
• Host: Goat
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Immunoprecipitation
  • SDS-Page
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1.1 mg/ml
• Purity: Ion exchange chromatography

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.