Human IgG Fc Isotype Control

• Reactivity: Hu
• Applications: ELISA, IP
• Clonality: Polyclonal
• Host: Human
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Human IgG Fc Isotype Control Summary

• Description: A human expressed protein corresponding to IgGFc.
• Preparation Method: Purified normal human IgG is isolated using fractionation and DEAE chromatography. Fc fragments were produced by papain digestion and affinity chromatography.
  
  Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  
  By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for human IgGFc, 2) not react with antiserum specific for IgA, IgM, kappa or lambda chain and 3) produce a single precipitin arc with antiserum against human serum identical to that produced with antiIgG-Fc.
• Source: Human Plasma
• Isotype: IgG
• Clonality: Polyclonal
• Host: Human
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
• Application Notes: Serum or plasma from donor has been tested and found negative for HIV-1 antigen, antibodies for HIV and HCV, and non-reactive for HBsAg, HIV-1 RNA, and HCV RNA. Not tested for AntiHBc. HANDLE AS IF CAPABLE OF TRANSMITTING INFECTIOUS AGENTS.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Phosphate Buffered Saline
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Notes

Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for human IgGFc, 2) not react with antiserum specific for IgA, IgM, kappa or lambda chain and 3) produce a single precipitin arc with antiserum against human serum identical to that produced with antiIgG-Fc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Isotype Control (NBP2-47132)(1)

Publication using NBP2-47132

• Vogl T, Klompus S, Leviatan S Et Al. Population-wide diversity and stability of serum antibody epitope repertoires against human microbiota Nature medicine 2021-07-19 [PMID: 34282338]