Rat IgG Fc Isotype Control

SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-PAGE of Rat IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c) and Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.

SDS-Page: Rat IgG Fc Isotype Control [NBP1-96967] - SDS-Page of Rat IgG F(c). Lane 1: Rat Fc - Non-reduced. Lane 2: Rat Fc - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 25 kDa, 25 kDa for Reduced Fc. Other band(s): None.

• Reactivity: Rt
• Applications: WB, ELISA, IHC, PAGE
• Host: Rat
• Conjugate: Unconjugated
• Format: Purified

Rat IgG Fc Isotype Control Summary

• Description: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c)
  
  Store vial at 4C prior to opening. Stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Specificity: No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain.
• Isotype: IgG
• Host: Rat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.