Goat anti-Mouse IgG Fc Secondary Antibody (Pre-adsorbed)

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody (Pre-adsorbed) [NBP2-61925] - Analysis of mouse tissue using goat anti-mouse DyLight 649 conjugated secondary antibody. Image from verified customer review. Image from the DyLight 650 version of this antibody.

Flow Cytometry: Goat anti-Mouse IgG Fc Secondary Antibody (Pre-adsorbed) [NBP2-61925] - Analysis of human osteosarcoma cells using goat anti-mouse DyLight 649 conjugated secondary antibody. Image from verified customer review. Image from the DyLight 650 version of this antibody.

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody (Pre-adsorbed) [NBP2-61925] - Analysis of c-Myc in human osteosarcoma cells using goat anti-mouse DyLight 649 conjugated secondary antiboy. Image from verified customer review. Image from the DyLight 650 version of this antibody.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: Pre-adsorbed
• Concentration: 0.5 mg/ml

Goat anti-Mouse IgG Fc Secondary Antibody (Pre-adsorbed) Summary

• Description: This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
• Immunogen: Mouse IgG
• Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. Less than 1% cross reactivity to human and rat IgG was detected.This antibody may cross react with IgG from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:20000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:200 - 1:2000
  • Western Blot 1:1000 - 1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Antigen Affinity-purified

Notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.