Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed)

Immunocytochemistry/Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) [NBP1-51898] - Analysis of c-Myc in human osteosarcoma cells using goat anti-mouse DyLight 649 conjugated secondary antiboy. Image from verified customer review.

Immunohistochemistry-Frozen: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) [NBP1-51898] - Analysis of mouse tissue using goat anti-mouse DyLight 649 conjugated secondary antibody. Image from verified customer review.

Flow Cytometry: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) [NBP1-51898] - Analysis of human osteosarcoma cells using goat anti-mouse DyLight 649 conjugated secondary antibody. Image from verified customer review.

• Reactivity: Mu
• Applications: Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: DyLight 650
• Format: Pre-adsorbed
• Concentration: 0.5 mg/ml

Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 650] (Pre-adsorbed) Summary

• Description: DyLight 649 is excited at 646 (in PBS) and emits at 674 (in PBS).
• Immunogen: Mouse IgG
• Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50 - 1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS containing 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for IgG Fc Antibody (NBP1-51898) (4)

Average Rating: 4

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Mouse.

reviewed by: Arun Satelli IHC-Fr Mouse 10/23/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse tissue
• Species: Mouse

reviewed by: Arun Satelli IHC-P Human 10/23/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human pancreatic tissue
• Species: Human

reviewed by: Arun Satelli ICC Human 10/23/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: Human osteosarcoma cell line
• Species: Human

reviewed by: Arun Satelli Flow Human 10/23/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Human osteosarcoma cell line
• Species: Human