Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 649] (Pre-absorbed)

Images

 
Immunocytochemistry/ Immunofluorescence: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 649] [NBP1-51898] - analysis of c-Myc in human osteosarcoma cells using goat anti-mouse DyLight 649 conjugated secondary ...read more
Immunohistochemistry-Frozen: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 649] [NBP1-51898] - analysis of mouse tissue using goat anti-mouse DyLight 649 conjugated secondary antibody. Image from verified customer ...read more
Flow Cytometry: Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 649] [NBP1-51898] - analysis of human osteosarcoma cells using goat anti-mouse DyLight 649 conjugated secondary antibody. Image from verified customer ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
DyLight 649
Format
Pre-absorbed
Concentration
0.5 mg/ml

Order Details

Goat anti-Mouse IgG Fc Secondary Antibody [DyLight 649] (Pre-absorbed) Summary

Immunogen
Mouse IgG-Fc Fragment
Specificity
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and rat immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:50 - 1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:500
Application Notes
IHC-Frozen application verified customer review.
Reviewed Applications
Read 4 Reviews rated 4
using
NBP1-51898 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS containing 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

DyLight 649 is excited at 646 (in PBS) and emits at 674 (in PBS).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for IgG Fc Antibody (NBP1-51898) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.
We have 4 reviews tested in 4 application: IHC-Fr, IHC-P, ICC, Flow.

Reviews using NBP1-51898:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
ICC
(1)
Flow
(1)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen IgG Fc NBP1-51898
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reviewed by:
Arun Satelli
IHC-Fr Mouse 10/23/2014
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse tissue
SpeciesMouse
CommentsHere, I tested the green filter also to see if there is non-specific seepage, and as we can see there is only background staining, nothing from the 649. This works great here.

Blocking

Blocking DetailsFish Gelatin

Details

Detection NotesDetection using fluoroscent microscope
Fixation DetailsRegular fixation of IHC frozen sections

Comments

CommentsHere, I tested the green filter also to see if there is non-specific seepage, and as we can see there is only background staining, nothing from the 649. This works great here.
Immunohistochemistry-Paraffin IgG Fc NBP1-51898
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reviewed by:
Arun Satelli
IHC-P Human 10/23/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman pancreatic tissue
SpeciesHuman
CommentsI was detecting a stromal marker and a epthileal marker in this tissue section. Red is stromal marker that is the 649 and seems no overlap between the two. Some of them show dual staining as expected. I would say very good, however just comparable to other dyes.

Blocking

Blocking DetailsFish Gelatin

Primary Anitbody

Dilution Ratio1:500

Details

Detection NotesDetection using confocal microscope
Fixation DetailsRegular fixation of IHC paraffin tissue
Wash Description5 min each 3 times

Comments

CommentsI was detecting a stromal marker and a epthileal marker in this tissue section. Red is stromal marker that is the 649 and seems no overlap between the two. Some of them show dual staining as expected. I would say very good, however just comparable to other dyes.
Immunocytochemistry IgG Fc NBP1-51898
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reviewed by:
Arun Satelli
ICC Human 10/23/2014
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman osteosarcoma cell line
SpeciesHuman
CommentsGood intense staining observed, I used 3 colors and there was no overlap between each of the colors, I used dapi, 488, 649.

Blocking

Blocking Details0.25% FBS in PBS

Primary Anitbody

Dilution Ratio1:500

Details

Detection NotesDetection using confocal microscope LSM510
Fixation DetailsCells were fixed in 4% PFA, PBS, Overnight 4 deg C
Wash Description5 min each 3 times in PBS

Comments

CommentsGood intense staining observed, I used 3 colors and there was no overlap between each of the colors, I used dapi, 488, 649.
Flow Cytometry IgG Fc NBP1-51898
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reviewed by:
Arun Satelli
Flow Human 10/23/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman osteosarcoma cell line
SpeciesHuman
CommentsDetection was done using a blue laser at BL3 filter, however if no compensation is done, seems like there is some seepage into the BL2 filter too at 1:50 dilution. After compensation there is no problem. Compensation was using beads.

Primary Anitbody

Dilution Ratio1:100

Details

Detection NotesDetection using flow cytometer
Fixation DetailsFixing using 4% PFA, Block in 0.25% FBS,
Permeabilization Detailsno permeabilization
Wash Description5 min each 3 times in PBS

Comments

CommentsDetection was done using a blue laser at BL3 filter, however if no compensation is done, seems like there is some seepage into the BL2 filter too at 1:50 dilution. After compensation there is no problem. Compensation was using beads.

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Recent Reviews

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Arun Satelli
10/23/2014
Application: IHC-Fr
Species: Mouse

Arun Satelli
10/23/2014
Application: IHC-P
Species: Human

Arun Satelli
10/23/2014
Application: ICC
Species: Human