Goat anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase]

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alkaline Phosphatase
• Concentration: 1.5 mg/ml

Goat anti-Human IgG Fc Secondary Antibody [Alkaline Phosphatase] Summary

• Description: Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
  
  Store undiluted liquid at 2-8C. For storage at -20C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
• Immunogen: Purified Human IgG, Fc fragment
• Specificity: Based on IEP, this antibody reacts with heavy gamma chains on human IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200-1:5000
• Application Notes: This conjugate is suitable for all immunoassay applications.

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, and Human IgG F(ab)'2 fragment

Packaging, Storage & Formulations

• Storage: Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
• Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free
• Preservative: 0.05% Sodium Azide
• Concentration: 1.5 mg/ml
• Purity: Affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Human IgG, Fc Antibody (NBP1-74975)(1)

Publication using NBP1-74975

• Herranen A. Unraveling the pleomorphic forms of Borrelia burgdorferi Thesis et al. 2014-01-01 (ELISA, Human)

Bioinformatics

• Uniprot:
  • O75015()
  • O75015()
  • O75015()