Rabbit anti-Bovine IgG Fc Secondary Antibody [Biotin]

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
LYOPH

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Rabbit anti-Bovine IgG Fc Secondary Antibody [Biotin] Summary

Immunogen
Bovine IgG  F(c) fragment
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum.  No reaction was observed against Bovine IgG F(ab')2.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
Application Notes
This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:2,000 - 1:15,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reacts with Bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Label Molecular Weight: 455, Restore with 1.0 mL of deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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