![ELISA: Rabbit anti-Bovine IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72630] - ELISA Results of Purified Rabbit anti-Bovine IgG Fc Secondary antibody [Alkaline Phosphatase] tested against purified Bovine IgG Fc Alk Phos. Each well was coated in duplicate with 1.0 ug of Bovine IgG Fc. The working dilution is 1:5300. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.](http://images.novusbio.com/fullsize/Rabbit-anti-Bovine-IgG-Fc-Secondary-Antibody-[Alkaline-Phosphatase]-ELISA-NBP1-72630-img0001.jpg "ELISA: Rabbit anti-Bovine IgG Fc Secondary Antibody [Alkaline Phosphatase] [NBP1-72630] - ELISA Results of Purified Rabbit anti-Bovine IgG Fc Secondary antibody [Alkaline Phosphatase] tested against purified Bovine IgG Fc Alk Phos. Each well was coated in duplicate with 1.0 ug of Bovine IgG Fc. The working dilution is 1:5300. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.")
| Reactivity | BvSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Alkaline Phosphatase |
| Description | Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine IgG, Bovine Serum and Bovine F(c) and no reaction against Bovine F(ab)2. |
| Immunogen | Rabbit anti-Bovine IgG Fc fragment Secondary Antibody was produced by repeated immunization with bovine IgG F(c) fragment in rabbit. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Dilutions |
|
| Application Notes | This product has been tested by ELISA. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/ml Polyethylene Glycol (PEG-8000) |
| Preservative | 0.09% Sodium Azide |
| Purity | Immunogen affinity purified |
Research Areas for IgG Fc Antibody (NBP1-72630)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
