Goat anti-Human IgG Fc Secondary Antibody [HRP]

Images

 
Western Blot: Goat anti-Human IgG Fc Secondary Antibody [HRP] [NB7449] - tested in human IgG. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

Goat anti-Human IgG Fc Secondary Antibody [HRP] Summary

Immunogen
Human IgG-Fc Fragment
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with human IgG. This may cross react with IgG from other species
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:50000
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/Immunofluorescence 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7449 in the following applications:

Publications
Read Publications using NB7449.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.1% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc Antibody (NB7449)(4)

Review for IgG Fc Antibody (NB7449) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB7449:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot IgG Fc NB7449
Enlarge
reviewed by:
Anonymous
WB Human 03/12/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman IgG
SpeciesHuman
LotP84

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution Rationo primary antibody used

Secondary Antibody

Secondary DescriptionGoat anti-human IgG-FC HRP, Novus NB7449 Lot# P84
Secondary Manufacturer Cat#NB7449
Secondary Concentration1:5000

Details

Detection Notesvery strong signal, clear background, exposure of 10 seconds is enough

Comments

CommentsThis is a clean secondary antibody, has very little back ground. It did not pick up light chain in my western blot.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Fc Antibody (NB7449) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG Fc Products

Blogs on IgG Fc

There are no specific blogs for IgG Fc, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
03/12/2015
Application: WB
Species: Human