IgG F(ab) Native Protein

SDS-Page: IgG F(ab) Native Protein [NBP1-97014] - SDS-PAGE of Rat IgG Fab Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.

Product Discontinued

IgG F(ab) Native Protein Summary

• Description: Native Rat IgG, Fab.

  Source: Serum

• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(ab')2. No reaction was observed against anti-Rat IgG F(c) or anti- Papain.
• Source: Rat Spleen
• Protein/Peptide Type: Native Protein
• Purity: Immunoelectrophoresis (IEP)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation
  • SDS-Page
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 5.73 mg/ml
• Purity: Immunoelectrophoresis (IEP)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.