Rabbit anti-Feline IgG F(ab')2 Secondary Antibody [Rhodamine]

• Reactivity: Fe
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Rhodamine
• Concentration: LYOPH

Rabbit anti-Feline IgG F(ab')2 Secondary Antibody [Rhodamine] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgG, Cat IgG F(ab')2 and Cat Serum
• Immunogen: Cat IgG F(ab')2 fragment
• Specificity: No reaction was observed against Cat IgG F(c).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
• Application Notes: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.