Mouse anti-Human IgG Secondary Antibody (4D2D9G8)

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Western Blot: Human IgG Antibody (4D2D9G8) [NBP1-51523] - Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb (lane 3, 4) against human serum (lane 1, 3) and plasma (lane 2, 4).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
4D2D9G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Mouse anti-Human IgG Secondary Antibody (4D2D9G8) Summary

Immunogen
Human IgG was isolated from human sera and purified by chromatography.
Specificity
This antibody is reactive with whole Human IgG, and has no cross reactivity with Human IgM.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
Application Notes
This Human IgG (4D2D9G8) antibody is useful for Western blot and ELISA.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Mouse anti-Human IgG Secondary Antibody (4D2D9G8)

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

Monoclonal anti-Human IgG is derived from a hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, and has no cross reactivity with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG Antibody (NBP1-51523). (Showing 1 - 1 of 1 FAQ).

  1. I have a question about your IgG antibody NBP1-51523. Have you ever tested the cross reactivity of this antibody against IgE?
    • We have not yet tested the cross reactivity of this antibody against IgE.

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