Goat anti-Monkey IgA/IgG/IgM (H+L) Secondary Antibody [FITC]

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Product Details

Summary
Reactivity PmSpecies Glossary
Applications ELISA, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Goat
Conjugate
FITC
Concentration
LYOPH

Order Details

Goat anti-Monkey IgA/IgG/IgM (H+L) Secondary Antibody [FITC] Summary

Immunogen
Purified polyclonal monkey IgG, and IgA and IgM containing factions isolated from monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reactivity of the antiserum is directed to the major isotypes of the monkey immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal monkey plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained.
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
(In)direct immunofluorescence

Reactivity Notes

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with immunoglobulins in Serum of other old-world Monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with other species as has been observed for Chimpanzee and man.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute by by adding 1 ml sterile distilled water.

Notes

The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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