Goat anti-Rat IgA/IgG/IgM (H+L) Secondary Antibody [Biotin]

Product Discontinued

Goat anti-Rat IgA/IgG/IgM (H+L) Secondary Antibody [Biotin] Summary

• Immunogen: Goat anti-Rat IgA/IgG/IgM (H+L) Secondary Antibody [Biotin] was produced by repeated immunization with Rat IgG, IgA and IgM whole molecules in goat.
• Specificity: This reagent is suitable for the detection of all rat immunoglobulin subclasses, isotypes and chain combinations.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:150000
  • Immunohistochemistry 1:1000 - 1:10000
  • Western Blot 1:2000 - 1:20000
• Application Notes: This secondary antibody are suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 2.0 ml deionized water (or equivalent).

Notes

This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA, Rat IgG and Rat IgM

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.