Rabbit anti-Human IgA Heavy Chain Secondary Antibody

Western Blot: Rabbit anti-Human IgA Secondary Antibody (Pre-absorbed) [NBP1-72777] - Lane 1: Human IgA Load: 100 ng per lane Primary antibody: HUMAN IgA (alpha chain) Antibody at 1:1000 for 60 min at RT Secondary antibody: DyLight 649 goat anti-rabbit at 1:20,000 for 30 min at RT Block: 5% BLOTTO 30 min at RT Predicted/Observed size: 60and 28 kDa, 60 and 28 kDa

Dot Blot: Rabbit anti-Human IgA Heavy Chain Secondary Antibody [NBP1-72777] - Antigen: Human IgA. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image from the FITC version of this antibody.

• Reactivity: Hu
• Applications: WB, DB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rabbit anti-Human IgA Heavy Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Human IgA alpha heavy chain
• Specificity: No reaction was observed against other human heavy or light chain proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:5000 - 1:20000
  • Immunohistochemistry 1:250 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This product is tested for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.