Rabbit anti-Human IgA Secondary Antibody

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Western Blot: Rabbit anti-Human IgA Secondary Antibody [NBP1-72777] - Lane 1: Human IgA Load: 100 ng per lane Primary antibody: HUMAN IgA (alpha chain) Antibody at 1:1000 for 60 min at RT Secondary antibody: DyLight 649 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.9 mg/ml

Rabbit anti-Human IgA Secondary Antibody Summary

Immunogen
Human IgA alpha heavy chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgA and Human Serum. No reaction was observed against other human heavy or light chain proteins.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:5000 - 1:20000
  • Immunohistochemistry 1:250 - 1:1000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
Application Notes
ELISA, Western Blot, Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.9 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rabbit anti-Human IgA Secondary Antibody

  • IgA
  • Immunoglobulin A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Blogs on IgA.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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