Human Nuclear Antigen Antibody (235-1) - Azide and BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Human Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525] - Paraformaldehyde fixed HeLa Cells stained with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) ...read more
Immunohistochemistry-Frozen: Human Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525] - Acetone-fixed frozen human Tonsil stained with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) using ...read more
Flow Cytometry: Human Nuclear Antigen Antibody (235-1) - Azide and BSA Free [NBP2-34525] - An intracellular stain was performed on HeLa cells with Human Nuclear Antigen Antibody (235-1) NBP2-34525AF647 (blue) along with ...read more

Product Details

Summary
Reactivity Hu, Ch(-), Mu(-), Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC-Fr, IP
Clone
235-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Human Nuclear Antigen Antibody (235-1) - Azide and BSA Free Summary

Immunogen
Nuclei of human myeloid leukemia biopsy cells
Localization
Nuclear
Marker
Human Cell Marker
Specificity
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1.0ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0 ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Publications
Read Publication using
NBP2-34525 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Human Nuclear Antigen Antibody (NBP2-34525)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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