Human Erythrocyte Lysing Flow Cytometry Kit

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Flow Cytometry: Human Erythrocyte Lysing Flow Cytometry Kit [WL1000-NOV] - Scatter dot histogram of whole blood stained with anti anti-CD14-PE and anti-CD3-APC followed by treatment with Erythrocyte Lysing Reagent and ...read more
Flow Cytometry: Human Erythrocyte Lysing Flow Cytometry Kit [WL1000-NOV] - Light scatter dot histogram (FS - forward light scatter vs. SS-side light scatter) of whole unlysed blood.
Flow Cytometry: Human Erythrocyte Lysing Flow Cytometry Kit [WL1000-NOV] - Light scatter dot histogram (FS - forward light scatter vs. SS-side light scatter) of whole blood treated with Erythrocyte Lysing Reagent.

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Human Erythrocyte Lysing Flow Cytometry Kit Summary

Description
Human Erythrocyte Lysing Kit is designed to gently lyse erythrocytes from leukocyte cell preparations and stabilize the leukocytes with fixative for later analysis by flow cytometry. Cells exposed to the lysing reagent retain their natural light scattering and fluorescent staining characteristics by flow cytometry. This erythrocyte lysing method also has an advantage over other lysing methods in that the viability of the leukocytes is maintained. The fixative and lysing reagents are provided as separate reagents, thus allowing cells to be used in tissue culture following red blood cell lysis.

Features
  • Gently lyses erythrocytes
  • Stabilizes leukocytes for analysis by flow cytometry
  • Maintains natural light scattering and fluorescent staining properties of leukocytes
  • Maintains leukocyte viability for subsequent cell culture manipulation
Kit Type
Flow Cytometry Kit

Packaging, Storage & Formulations

Storage
Store at room temperature.

Kit Components

Components
  1. Fixative (10X)
  2. H-Lyse Buffer (10X)
  3. Wash Buffer (10X)

Background

The lysis of erythrocytes from whole blood is an important initial step in the isolation and analysis of enriched leukocyte preparations. Recovered immune cells can be accurately characterized following red blood cell removal. Lysis of erythrocytes under conditions that do not disrupt lymphocytes or myeloid cells is critical for downstream applications utilizing leukocytes harvested from whole blood.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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