HRP Antibody (2H11cc)

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Product Details

Summary
Reactivity All-NASpecies Glossary
Applications ELISA, IHC
Clone
2H11cc
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

HRP Antibody (2H11cc) Summary

Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified peroxidase from horseradish.
Specificity
Horseradish peroxidase isoforms with pI from 3.5 to 9.5. NB110-8035 does not influence enzymatic activity upon immune complex formation.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500

Reactivity Notes

Chemical

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for HRP Antibody (2H11cc)

  • EC 1.11.1.7
  • Horseradish Peroxidase
  • HPRC 1
  • HPRC1
  • HRPA2
  • HRPC 2
  • HRPC2
  • HRPE5
  • HRPN
  • Neutral peroxidase
  • Peroxidase A2
  • Peroxidase C1A
  • Peroxidase C1B
  • Peroxidase C2
  • Peroxidase C3
  • Peroxidase E5
  • Peroxidase N
  • PRXC1A
  • PRXC1B
  • PRXC2
  • PRXC3

Background

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Additional HRP Products

Array NB110-8035

Blogs on HRP.

Ultrasensitive IHC Detection with HRP-Polymer Conjugates
Signal amplification methods are widely used in immunohistochemistry (IHC) for detection of rare epitopes and low abundance antigens. While many of these techniques such as the avidin-biotin complex (ABC) method improve staining, they frequently re...  Read full blog post.

5 Stars for the Lightning-Link HRP Antibody Labeling Kit
Novus' Lightning-Link HRP Antibody Labeling Kit (cat# 701-0000) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "Fast, efficient, stable direct HRP conjugation - best reagent we have found..." Anot...  Read full blog post.

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