HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human HNSCC stained with HPV16E6 & HPV18E6 Mouse Monoclonal Antibody (HPV/16/18).

• Reactivity: V-Vi
• Applications: ICC/IF, IHC
• Clone: HPV16/1295 + HPV18/1297
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07675).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: HPV18 E6-beta-galactosidase fusion protein
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.5 - 1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1 - 2 ug/ml
• Application Notes: Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Reactivity Notes

Type 16 of Human Papilloma Virus (HPV-16)

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) - Azide and BSA Free

• E6
• Human Papilloma Virus
• Human papillomavirus type 16 E6
• Human papillomavirus type 18 E6
• Protein E6

Background

Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1, 6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation. Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.