HPa1 Antibody (DHIC2-2C12) - BSA Free

Immunocytochemistry/Immunofluorescence: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Immunofluorescence on frozen section of human pancreas.

Flow Cytometry: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Analysis using the DyLight 550 conjugate of NBP1-18949. Staining of KB, KB-VCR. Fixed in 4% paraformaldehyde.

Flow Cytometry: HPa1 Antibody (DHIC2-2C12) [NBP1-18949] - Analysis of enzyme dispersed human pancreas cells.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: DHIC2-2C12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

HPa1 Antibody (DHIC2-2C12) - BSA Free Summary

• Immunogen: Human pancreatic enriched alpha cells
• Localization: Cell surface
• Marker: Alpha Cell Marker
• Specificity: Specific for alpha endocrine cell types.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Western Blot 1:100-1:2000
• Application Notes: This HPa1 (DHIC2-2C12) antibody is useful for Immunohistochemistry on acetone fixed frozen sections, Immunocytochemistry/Immunofluorescence, Flow cytometry and Western blot.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for HPa1 Antibody (DHIC2-2C12) - BSA Free

• human pancreatic alpha cell

Background

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPa1 Antibody (NBP1-18949)(4)

Publications using NBP1-18949

• Casteels T, Bajew S, ReiniS J et al. SMNDC1 links chromatin remodeling and splicing to regulate pancreatic hormone expression Cell reports 2022-08-30 [PMID: 36044849] (FLOW)
  
  Details:
  Dilutions: 1:100
• Jakel C, Bergmann F, Toth R et al. Genome-wide genetic and epigenetic analyses of pancreatic acinar cell carcinomas reveal aberrations in genome stability Nat Commun. 2017-11-06 [PMID: 29109526] (FLOW, Human)
• Dorrell C, Schug J, Lin C et al. Transcriptomes of the major human pancreatic cell types. Diabetologia. 2011-01-01 [PMID: 21882062]
• Dorrell, et al. Isolation of major pancreatic cell types long-term culture-initiating cells using novel human surface markers. Stem Cell Rsch 1(3):183-194. 2008-01-01 [PMID: 19383399] (FLOW, IHC-Fr, Human)