HIV-1 gp120 ELISA Kit (Colorimetric)

ELISA: HIV-1 gp120 ELISA Kit (Colorimetric) [NBP2-80343] - Example standard curve for HIV-1 gp120 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Conjugate: HRP
• Suitable Sample Type: Recombinant protein
• Standard Curve Range: 34.38-2200 pg/ml (example only; lot dependent)
• Sensitivity: 14.47 pg/ml

HIV-1 gp120 ELISA Kit (Colorimetric) Summary

• Specificity: Recognizes both recombinant and natural HIV-1 gp120.
• Standard Curve Range: 34.38-2200 pg/ml (example only; lot dependent)
• Sensitivity: 14.47 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 4.53
• Intra-Assay: CV% < 3.1
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: env

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Alternate Names for HIV-1 gp120 ELISA Kit (Colorimetric)

• Envelope surface glycoprotein gp120
• gp120 glycoprotein
• gp120
• Human immunodeficiency virus 1
• SU
• Surface protein

Background

HIV-1 gp120 is a surface glycoprotein that enables viral attachment and entry into host cells. It is also involved in neurotoxicity and has been implicated in HIV-associated neurocognitive disorders. HIV-1 gp120 also induces apoptosis in neuronal cells by signaling mitochondrial-death proteins which may upregulate the death receptor, Fas. Because HIV gp120 is necessary for HIV-1 to enter CD4 cells, gp120 is one of the main targets of HIV vaccine research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HIV-1 gp120 ELISA Kit (NBP2-80343)(1)

Publication using NBP2-80343

• Zihni Onur Uygun, Savas Tasoglu Impedimetric antimicrobial peptide biosensor for the detection of human immunodeficiency virus envelope protein gp120. iScience 2024-03-15 [PMID: 38414859]

FAQs for HIV-1 gp120 ELISA Kit (NBP2-80343). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: env