HIV-1 Gag p24 Antibody (ND1) - BSA Free

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Immunocytochemistry/ Immunofluorescence: HIV-1 Gag p24 Antibody (ND1) - BSA Free [NB500-473] - Transfected p24 (green) was detected in Huh-7 cells. Cells were fixed with PFA 4%. Antibody concentration: 1:50. Dilution ...read more

Product Details

Summary
Reactivity VSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
ND1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

HIV-1 Gag p24 Antibody (ND1) - BSA Free Summary

Description
Novus Biologicals Mouse HIV-1 Gag p24 Antibody (ND1) - BSA Free (NB500-473) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-HIV-1 Gag p24 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
HIV-1 Gag p24 C-terminal peptide.
Specificity
The antibody reacts with HIV-1 Gag p24). Specificity was verified by Western Blotting in reducing conditions with Sanofi Pasteur NEW LAV-BLOT I kit. Epitope was reported to be located within the amino acids 51-110.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
gag
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
Application Notes
Purity is > 95% (by SDS-PAGE). Validated for ICC/IF from a verified customer review.
Publications
Read Publications using NB500-473.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate buffered saline (PBS), pH 7.4
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Multi-step

Alternate Names for HIV-1 Gag p24 Antibody (ND1) - BSA Free

  • CA
  • Capsid Protein
  • HIV1 Gag p24
  • HIV-1 Gag p24
  • Pr55(Gag)

Background

HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIV-1 Gag p24 Antibody (NB500-473)(7)

We have publications tested in 2 confirmed species: Human, Virus.


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Filter By Species
Human
(1)
Virus
(1)
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Showing Publications 1 - 7 of 7.
Publications using NB500-473 Applications Species
Adhikarimayum LS, Dylan S, Daniel N et al. Cocaine sensitizes the CD4+ T cells for HIV infection by co-stimulating NFAT and AP-1 iScience 2022-12-01 [PMID: 36483012]
Haleyur Giri Setty MK, Kurdekar A, Mahtani P et al. Cross-Subtype Detection of HIV-1 Capsid p24 Antigen Using a Sensitive Europium Nanoparticle Assay AIDS Research and Human Retroviruses 2019-04-01 [PMID: 30411969]
Turbe V, Gray ER, Lawson VE et al. Towards an ultra-rapid smartphone- connected test for infectious diseases Sci Rep 2017-09-20 [PMID: 28931860] (Virus) Virus
Eleanor R Gray, Jennifer C Brookes, Christophe Caillat, Valérian Turbé, Benjamin L J Webb, Luke A Granger, Benjamin S Miller, Laura E McCoy, Mohamed El Khattabi, C Theo Verrips, Robin A Weiss, Dorothy M Duffy, Winfried Weissenhorn, Rachel A McKendry Unravelling the Molecular Basis of High Affinity Nanobodies against HIV p24: In Vitro Functional, Structural, and in Silico Insights. ACS infectious diseases 2017-11-29 [PMID: 28591513]
Haleyur Giri Setty MK, Liu J, Mahtani P. Novel Time Resolved Fluorescence Europium Nano Particle Immunoassay for detection of HIV-1 Group O viruses using Micro-plate and Microchip Platforms AIDS Research and Human Retroviruses 2016-01-28 [PMID: 26978478]
Sung CH, Chuang JZ. The cell biology of vision. J Cell Biol 2010-09-20 [PMID: 20855501] (Human) Human
Tang S, Zhao J, Wang A et al. Characterization of immune responses to capsid protein p24 of human immunodeficiency virus type 1 and implications for detection. Clin Vaccine Immunol 2010-08-01 [PMID: 20534793]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol gag