Histone Nucleosome Core ELISA Kit (Colorimetric)

Product Discontinued

Histone Nucleosome Core ELISA Kit (Colorimetric) Summary

• Description: Nucleosome Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against Nucleosomes in human serum or plasma.
• Standard Curve Range: 12.5 to 200 U/mL
• Sensitivity: 0.5 U/mL
• Assay Type: Indirect ELISA
• Inter-Assay: CV% < 5.2-12.4%
• Intra-Assay: CV% < 3.1-6.4%
• Sample Volume: 100 uL (prediluted)
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification
• Application Notes: Useful for quantification.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Histone Nucleosome Core ELISA Kit (KA1091)(3)

Publications using KA1091

• Koupenova M, Corkrey HA, Vitseva O et al. The role of platelets in mediating a response to human influenza infection. Nat Commun. 2018-09-01 [PMID: 30992428] (ELISA)
  
  Details:
  Citation using the Azide Free form of this antibody.
• Yadav R, Yoo DG, Kahlenberg JM et al. Systemic levels of anti-PAD4 autoantibodies correlate with airway obstruction in cystic fibrosis. J Cyst Fibros. 2019-03-07 [PMID: 30638826]
• Yang Y, Wang Y, Zhao M et al. Tormentic acid inhibits IL-1beta-induced chondrocyte apoptosis by activating the PI3K/Akt signaling pathway. Mol Med Rep 2018-03-01 [PMID: 29328385] (ELISA)

FAQs for Histone Nucleosome Core ELISA Kit (KA1091). (Showing 1 - 2 of 2 FAQs).

1. Dose this kit measure Nucleosome in Molar Concentration? If it measures concentration in units, then is it possible to convert them in molar? Dose this kit comes with positive control?
   • This kit is produced by a Taiwanese company called Abnova and we distribute it for them. We do no in-house testing or development of their products. Full information for this kit can be found in their protocol.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.