His Tag Antibody (AD1.1.10R) [Alexa Fluor® 647]

HEK293 human embryonic kidney cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag Alexa Fluor® 647-conjugated Mono-clonal Antibody (Catalog # IC0501R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: All-Multi
• Applications: Flow
• Clone: AD1.1.10R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

His Tag Antibody (AD1.1.10R) [Alexa Fluor® 647] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: His-tagged peptide
• Specificity: Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags located at the amino- or carboxyl- terminus.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for His Tag Antibody (AD1.1.10R) [Alexa Fluor® 647]

• 6 His epitope tag
• 6-His Tag
• 6X His Tag
• 6X His
• 6x-His Tag
• 6X-His
• H
• Hexa His tag
• HHHHHH epitope tag
• HHHHHH tag
• His Tag
• HIS
• His6-Tag
• polyhistidine tag
• polyHistidine
• Poly-histidine

Background

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for His Tag Antibody (IC0501R)(2)

Publications using IC0501R

• Masloh, S;Chevrel, A;Culot, M;Perrocheau, A;Kalia, YN;Frehel, S;Gaussin, R;Gosselet, F;Huet, S;Zeisser Labouebe, M;Scapozza, L; Enhancing Oral Delivery of Biologics: A Non-Competitive and Cross-Reactive Anti-Leptin Receptor Nanofitin Demonstrates a Gut-Crossing Capacity in an Ex Vivo Porcine Intestinal Model Pharmaceutics 2024-01-16 [PMID: 38258126] (Flow Cytometry, Human)
• Saunders, N;Fernandez, I;Planchais, C;Michel, V;Rajah, MM;Baquero Salazar, E;Postal, J;Porrot, F;Guivel-Benhassine, F;Blanc, C;Chauveau-Le Friec, G;Martin, A;Grzelak, L;Oktavia, RM;Meola, A;Ahouzi, O;Hoover-Watson, H;Prot, M;Delaune, D;Cornelissen, M;Deijs, M;Meriaux, V;Mouquet, H;Simon-Lorière, E;van der Hoek, L;Lafaye, P;Rey, F;Buchrieser, J;Schwartz, O; TMPRSS2 is a functional receptor for human coronavirus HKU1 Nature 2023-10-25 [PMID: 37879362] (Flow Cytometry, Human)

Review for His Tag Antibody (IC0501R) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 03/14/2019

Summary

• Application: Flow Cytometry
• Sample Tested: 3 human cancer cell lines,cancer cell line
• Species: Human
• Lot: AFCX0318101