His Tag Antibody (AD1.1.10R) [Phycoerythrin]

In a functional flow cytometry test, recombinant SARS-CoV2 S1 RBD His-tagged protein (50 ng/mL, 10500-CV) binds to HEK293 cells transfected with Human ACE-2 and eGFP. (A) Protein binding was detected with Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) or Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) alone. View our protocol for View our protocol for Staining Membrane-associated Proteins.

• Reactivity: All-Multi
• Applications: Flow
• Clone: AD1.1.10R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

His Tag Antibody (AD1.1.10R) [Phycoerythrin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: His-tagged peptide
• Specificity: Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags located at the amino- or carboxyl- terminus
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for His Tag Antibody (AD1.1.10R) [Phycoerythrin]

• 6 His epitope tag
• 6-His Tag
• 6X His Tag
• 6X His
• 6x-His Tag
• 6X-His
• H
• Hexa His tag
• HHHHHH epitope tag
• HHHHHH tag
• His Tag
• HIS
• His6-Tag
• polyhistidine tag
• polyHistidine
• Poly-histidine

Background

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.