His Tag Antibody (33D10.D2) [DyLight 405] Summary
This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Protein A purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Western Blot 1:2000-1:5000
- ELISA 1:20000-1:1000000
- Fluorophore-linked immunosorbent assay 1:100-1:2000
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01% Sodium Azide
Protein A purified
Reconstitute with 0.1 ml deionized water.
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free Color: Blue Excitation Wavelength: 400 Emission Wavelength: 420 Conjugation Chemistry: N-hydroxysuccinimide (NHS) ester. Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for His Tag Antibody (33D10.D2) [DyLight 405]
- 6 His epitope tag
- 6X His
- HHHHHH epitope tag
- HHHHHH tag
- His Tag
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
Publications for His Tag Antibody (NBP1-97328) (0)
There are no publications for His Tag Antibody (NBP1-97328).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for His Tag Antibody (NBP1-97328) (0)
There are no reviews for His Tag Antibody (NBP1-97328).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for His Tag Antibody (NBP1-97328) (0)
Other Available Formats
Additional His Tag Products
Blogs on His Tag