His Tag Antibody (33D10.D2) [HRP]

Images

 
Western Blot: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression ...read more
ELISA: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and ...read more
ELISA: 6-Histidine Epitope Tag Antibody (33D10.D2) [HRP] [NBP1-42783] - Antigen: 6X HIS-tagged conjugated BSA at the N or C terminus of the 6XHis. Coating amount: 0.15ug per welll. Primary antibody (direct detection): ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
33D10.D2
Clonality
Monoclonal
Host
Mouse
Conjugate
HRP
Concentration
LYOPH

Order Details

His Tag Antibody (33D10.D2) [HRP] Summary

Immunogen
This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:10000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Readout System
Publications
Read Publication using NBP1-42783.

Reactivity Notes

6x His tag specific

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water to a final concentration of 1.0 mg/ml.

Alternate Names for His Tag Antibody (33D10.D2) [HRP]

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for His Tag Antibody (NBP1-42783)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for His Tag Antibody (NBP1-42783). (Showing 1 - 1 of 1 FAQ).

  1. I'm planning to use the 6-Histidine Epitope Tag Antibody for immunohistochemistry (formalin fixed, paraffin embedded) and am looking for a suitable positive control. Does Novus supply or recommend any positive controls which would be suitable?
    • This antibody is to the epitope tag, so there really isn't anything we can offer as a positive control. Any sample confirmed to have the His-tag would probably be your best bet as a positive control.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-42782

Additional His Tag Products

His Tag NBP1-42783

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