His Tag Antibody (33D10.D2)

Images

 
Western Blot: His Tag Antibody (33D10.D2) [NBP1-42781] - Analysis using Biotin conjugate of NBP1-42781. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing ...read more
Flow Cytometry: His Tag Antibody (33D10.D2) [NBP1-42781] - Analysis using HRP conjugate of NBP1-42781. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing ...read more
Western Blot: His Tag Antibody (33D10.D2) [NBP1-42781] - Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng ...read more
Western Blot: His Tag Antibody (33D10.D2) [NBP1-42781] - Detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.
Flow Cytometry: His Tag Antibody (33D10.D2) [NBP1-42781] - Analysis using the DyLight 405 conjugate of NBP1-42781.
Flow Cytometry: His Tag Antibody (33D10.D2) [NBP1-42781] - Analysis using the DyLight 488 conjugate of NBP1-42781.
ELISA: His Tag Antibody (33D10.D2) [NBP1-42781] - ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged construct, GST- and RON-tagged purified ...read more
ELISA: His Tag Antibody (33D10.D2) [NBP1-42781] - Analysis using the HRP conjugate of NBP1-42781. Antigen: 6X HIS-tagged conjugated BSA at the N or C terminus of the 6XHis. Coating amount: 0.15ug per welll. Primary ...read more
ELISA: His Tag Antibody (33D10.D2) [NBP1-42781] - Analysis of Biotin conjugate of NBP1-42781. ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged ...read more
ELISA: His Tag Antibody (33D10.D2) [NBP1-42781] - Analysis of HRP conjugate of NBP1-42781. ELISA of Mouse anti-6xHIS Tag Antibody. Antigen: HIS-tagged purified protein and E. coli cell lysates expressing HIS-Tagged ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
33D10.D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates

His Tag Antibody (33D10.D2) Summary

Immunogen
This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:75000
  • Flow Cytometry
  • Immunohistochemistry 1:10 - 1:500
Application Notes
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used - identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either - the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Publications
Read Publication using NBP1-42781.

Reactivity Notes

6x His tag specific

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for His Tag Antibody (33D10.D2)

  • 6 His epitope tag
  • 6X His
  • 6X-His
  • H
  • HHHHHH epitope tag
  • HHHHHH tag
  • His Tag
  • HIS
  • polyHistidine
  • Poly-histidine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for His Tag Antibody (NBP1-42781)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-42782
DyLight 405 Labeled NBP1-97328
DyLight 488 Labeled NBP1-97329
DyLight 680 Labeled NBP1-97332
HRP Labeled NBP1-42783

Additional His Tag Products

His Tag NBP1-42781

Research Areas for His Tag Antibody (NBP1-42781)

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