Herpes Simplex Virus 1 Antibody (HSVI/2095)

Immunohistochemistry-Paraffin: Herpes Simplex Virus 1 Antibody (HSVI/2095) [NBP3-07669] - Formalin-fixed, paraffin-embedded human Cervix stained with Herpes Simplex Virus 1 Mouse Monoclonal Antibody (Herpes Simplex Virus 1/2095).

• Reactivity: V-Vi
• Applications: IHC
• Clone: HSVI/2095
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Herpes Simplex Virus 1 Antibody (HSVI/2095) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08465)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Baculovirus-expressed Herpes Simplex Virus 1 DNA polymerase (POL) and POL/UL42 complex
• Localization: Cytoplasmic and/or Nuclear
• Specificity: The antibody reacts with HSV type 1 specific antigen. It is suitable for detection of HSV in human cellular material obtained from superficial lesions or biopsies and for the early identification of HSV in infected tissue cultures. The herpes simplex virus (HSV) (also known as cold sore, night fever or fever blister) is a virus that causes a contagious disease. There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The HSV-1 strain generally appears in the orafacial organs. HSV2 usually resides in the sacral ganglion at the base of the spine. All herpes viruses are morphologically identical: they have a large double-stranded DNA genome and the virion consists of an icosahedral nucleo-capsid, which is surrounded by a lipid bilayer envelope. UL42, the processivity subunit of the HSV-1DNA polymerase binds DNA as a monomer and is essential for the replication of the virus. UL42 reduces the rate of dissociation from primer-template DNA, but it does not reduce the rate of elongation. UL42 increases the ability of UL9 to load onto DNA, thus increasing its assembly into a functional complex that is capable of unwinding duplex DNA.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

HSV1 (Herpes Simplex Virus 1)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Herpes Simplex Virus 1 Antibody (HSVI/2095)

• Herpes simplex virus 1
• Herpes simplex virus type 1
• Herpes simplex virus
• HSV 1
• Human herpesvirus 1
• Human herpesvirus type 1

Background

Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.