Herpes Simplex Virus 1 Antibody (HSVI/2045)

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Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB
Clone
HSVI/2045
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Herpes Simplex Virus 1 Antibody (HSVI/2045) Summary

Immunogen
Baculovirus-expressed Herpes Simplex Virus 1 DNA polymerase (POL) and POL/UL42 complex
Localization
Cytoplasmic and/or Nuclear
Specificity
The antibody reacts with HSV type 1 specific antigen. It is suitable for detection of HSV in human cellular material obtained from superficial lesions or biopsies and for the early identification of HSV in infected tissue cultures. The herpes simplex virus (HSV) (also known as cold sore, night fever or fever blister) is a virus that causes a contagious disease. There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The HSV-1 strain generally appears in the orafacial organs. HSV2 usually resides in the sacral ganglion at the base of the spine. All herpes viruses are morphologically identical: they have a large double-stranded DNA genome and the virion consists of an icosahedral nucleo-capsid, which is surrounded by a lipid bilayer envelope. ICP8, the HSV1 encoded single-strand DNA (ssDNA)-binding protein, is the major DNA binding protein of HSV1. ICP8 promotes single-stranded DNA to assemble into a homologous duplex plasmid producing a displacement loop. At higher concentrations, however, ICP8 facilitates the reverse reaction due to its helix destabilizing activity.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
150 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

HSV1 (Herpes Simplex Virus 1)

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08639)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Herpes Simplex Virus 1 Antibody (HSVI/2045)

  • Herpes simplex virus 1
  • Herpes simplex virus type 1
  • Herpes simplex virus
  • HSV 1
  • Human herpesvirus 1
  • Human herpesvirus type 1

Background

Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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