Hepatocyte Specific Antigen Antibody (SPM582)

Immunohistochemistry-Paraffin: Hepatocyte Specific Antigen Antibody (SPM582) [NBP2-32859] - Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with HSA Monoclonal Antibody (SPM582).

• Reactivity: Hu, Ca
• Applications: ICC/IF, IHC
• Clone: SPM582
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Hepatocyte Specific Antigen Antibody (SPM582) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34814)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Extract of a formalin-fixed, rejected-allograft of a human liver
• Localization: Finely granular cytoplasmic
• Marker: Hepatocellular Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Hepatocyte Specific Antigen Antibody (SPM582)

• Hepatocyte
• HSA

Background

HSA (Human Serum Albumin) is useful in studying hepatocellular tumors. It may be useful in differentiating clear cell hepatocellular carcinomas from other clear cell malignancies. It has been shown in the literature to be useful in differentiating hepatoblastoma of embryonal type from small round cell tumors of childhood. It labels an antigen in the mitochondrial fraction of the liver homogenates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hepatocyte Specific Antigen Antibody (NBP2-32859)(1)

Publication using NBP2-32859

• Lee HM, Joh JW, Seo SR, Kim WT et al. Cell-surface major vault protein promotes cancer progression through harboring mesenchymal and intermediate circulating tumor cells in hepatocellular carcinomas. Sci Rep. 2017-10-16 [PMID: 29038587] (ICC/IF, Human)
  
  Details:
  Citation using the DyLight 650 form of this antibody.