Recombinant Hepatitis C Virus His Protein

SDS-Page: Hepatitis C Virus Protein [NBP1-41149] - HCV NS3, 27.9 kDa (268aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Hepatitis C Virus His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1225-1456 of HCV Hepatitis C Virus
  
  

  Source: E.coli

  Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 27.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Hepatitis C Virus

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Hepatitis C Virus His Protein

• HCV NS5
• Hepatitis C virus non structural protein 5
• Hepatitis C virus nonstructural protein 5
• Hepatitis C Virus NS5

Background

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.