Recombinant Virus Hepatitis C Virus Protein

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SDS-Page: Hepatitis C Virus Protein [NBP1-41149] - HCV NS3, 27.9 kDa (268aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity ViSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Virus Hepatitis C Virus Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1225-1456 of HCV Hepatitis C Virus

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSVAHL HAPTGSGKST KVPAAYAAQG YKVLVLNPSV AATLGFGAYM SKAHGVDPNI RTGVRTITTG SPITYSTYGK FLADGGCSGG AYDIIICDEC HSTDATSILG IGTVLDQAET AGARLVVLAT ATPPGSVTVS HPNIEEVALS TTGEIPFYGK AIPLEVIKGG RHLIFCHSKK KCDELAAKLV ALGINAVAYY RGLDVSVIPT SGDVVVVSTD ALMTGFTGDF DSVIDCNT

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
HCVgp1
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
27.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Virus Hepatitis C Virus Protein

  • HCV NS5
  • Hepatitis C virus non structural protein 5
  • Hepatitis C virus nonstructural protein 5
  • Hepatitis C Virus NS5

Background

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. Nonstructural (NS) proteins of HCV play major roles in viral replication and the pathogenesis of liver diseases. The NS3 protein of hepatitis C virus (HCV) contains protease and RNA helicase activities, both of which are likely to be essential for HCV propagation. Recombinant HCV NS3 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol HCVgp1