Hepatitis B Virus Antibody

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Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5.0 mg/ml

Order Details

Hepatitis B Virus Antibody Summary

Immunogen
Synthetic peptide corresponding to the amino acids 21-47 of the native molecule conjugated to Keyhole limpet Haemocyanin.
Specificity
Recognises the 39 and 41 kD (L-protein) in Blotting. Both bands are peptide blockable.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:500
  • ELISA 1:100-1:2000
Publications
Read Publication using NB100-66430.

Reactivity Notes

Viral. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.1% Sodium Azide
Concentration
5.0 mg/ml
Purity
Protein G purified

Alternate Names for Hepatitis B Virus Antibody

  • HBsAg
  • Hepatitis B Receptor Binding Fragment Pre S

Background

Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hepatitis B Virus Antibody (NB100-66430)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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