HeLa Whole Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
2 mg/ml

Order Details

HeLa Whole Cell Lysate Summary

Specificity
HeLa-S3 (Human Cervix Epithelioid Carcinoma)

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Supplied in SDS sample buffer containing 5% Beta-mercaptoethanol.
Concentration
2 mg/ml

Lysate Details for HeLa

Type
Cell
Tissue
Skin
Subcellular Fraction
Whole

Notes

GROWTH MEDIA: Jokliks-MEM and 5% NCS (newborn calf serum)

Background

HeLa lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HeLa lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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WB Video Protocol

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